4-hydroperoxy cyclophosphamide

Research use only, not for human use.

4-hydroperoxy cyclophosphamide (4-OOH-CY) is an active metabolite of Cyclophosphamide that induces hepatotoxicity.

4-Hydroperoxy cyclophosphamide is the active metabolite form of the precursor Cyclophosphamide. 4-Hydroperoxy cyclophosphamide cross-links DNA to induce T cell apoptosis independent of caspase receptor activation, and can activate the mitochondrial death pathway by producing reactive oxygen species (ROS). 4-Hydroperoxy cyclophosphamide can be used in the study of rheumatoid arthritis and autoimmune diseases.

Western Blot Analysis Cell Line:CTL Concentration:3 μg/mL Incubation Time:24, 48, 72 hResult:Increased the expression of p53 and Bax after 24 h.Apoptosis Analysis Cell Line:Jurkat, CTL Concentration:1 or 3 μg/mL Incubation Time:24 , 48 , 72h Result: Caspase-8 deficiency prevented CD95-mediated death induced by anti-CD95 monoclonal antibody (mAb) APO-1.Overexpression of Bcl-2 inhibited apoptosis. Induced cell death proceeded independently of caspase inhibition.

Animal Model:BALB/c mice Dosage:200 mg/kg Administration:i.p.Result:Exhibited a marked depletion of immature double-positive CD4 t CD8 t thymocytes and mature single-positive CD4+ and CD8+ T cells.

——

Others

Other Targets

Reactive Oxygen Species | DNA Alkylator/Crosslinker | Apoptosis | Drug Metabolite

——

——

39800-16-3

293.09

C7H15Cl2N2O4P

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (170.60 mM; Ultrasonic )

O=P1(OCCC(OO)N1)N(CCCl)CCCl

——

(-20℃)

With Ice Pack

≥ 2 years